Adele Addington, PhD
- Research Associate
- Focus area(s): Molecular signaling during exercise
Education
Ph.D., Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA, 1994
B.S., Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg, VA, 1990
Experience
August 2018 — present: Laboratory Manager, Dr. Siobhan Craige, Department of Human, Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA
August 2014 — August 2018: Project/Laboratory Manager, Dr. Matt Hulver/Dr. Robert Grange, Metabolic Phenotyping Core/Department of Human, Nutrition, Foods and Exercise, Virginia Tech, Blakcsburg, VA
August 2008 — August 2014: Laboratory Manager, Dr. Susan Hutson, Department Human, Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, VA
August 2003 — May 2008: Assistant Professor, Department of Chemistry, Roanoke College, Salem, VA
August 1996 — August 2003: Instructor and Assistant Professor, Department of Chemistry, Virginia Military Institute, Lexington, VA
February 2006 — August 2006: Postdoctoral Research Associate / Adjunct Research Faculty, Dr. Lloyd Wolfinbarger, Center for Biotechnology, Old Dominion University, Norfolk, VA
October 1995 — January 1996: Postdoctoral Research Associate, Dr. Gary F. Clark, Department of Biochemistry, Eastern Virginia Medical School, Norfolk, VA
June 1994 — September 1995: Postdoctoral Research Associate, Dr. David A. Johnson, Department of Biochemistry, Quillen College of Medicine, East Tennessee State University, Johnson City, TN
Program Focus
The Craige Lab is interested in the immediate responses to exercise, and how the various cells within a tissue communicate to coordinate the appropriate response to the exercise / stress stimulus. We are interested in the biochemical signaling that occurs between endothelial cells and myocytes in skeletal muscle during exercise and during the recovery phase. Our work includes rodent-based studies, protein and nucleic acid extraction and subsequent analysis by qPCR and western blotting. We also use a variety of cultured cells to help elucidate the molecular intercellular communication between endothelial cells and myocytes in response to stress stimuli.
As the Laboratory Manager / Research Associate for Dr. Craige, I assist our students in their work, ensure that materials and reagents are available, and manage our mouse colony. I assist in preparing research proposals and manuscripts for publication. I establish working experimental protocols and also compose and obtain regulatory permissions for the lab. I also complete specific research projects pertinent to the lab’s overall research goals.
Reviewed Journals
- Hamm SE, Fathalikhani DD, Bukovec KE, Addington AK, Zhang H, Perry JB, McMillan RP, Lawlor MW, Prom MJ, Vanden Avond MA, Kumar SN, Coleman KE, Dupont JB, Mack DL, Brown DA, Morris CA, Gonzalez JP, Grange RW. (2021) Voluntary wheel running complements microdystrophin gene therapy to improve muscle function in mdx mice. Mol Ther Methods Clin Dev. 2021 Jun 11;21:144-160. doi: 10.1016/j.omtm.2021.02.024.
- Specht KS, Kant S, Addington AK, McMillan RP, Hulver MW, Learnard H, Campbell M, Donnelly SR, Caliz AD, Pei Y, Reif MM, Bond JM, DeMarco A, Craige B, Keaney JF Jr, Craige SM. (2021) Nox4 mediates skeletal muscle metabolic responses to exercise. Mol Metab. 2021 Mar;45:101160. doi: 10.1016/j.molmet.2020.101160.
- Griffin LE, Fausnacht DW, Tuzo JL, Addington AK, Racine KC, Zhang H, Hughes MD, England KM, Bruno RS, O'Keefe SF, Neilson AP, Stewart AC. (2019) Flavanol supplementation protects against obesity-associated increases in systemic interleukin-6 levels without inhibiting body mass gain in mice fed a high-fat diet. Nutr Res. 2019 Jun;66:32-47. doi: 10.1016/j.nutres.2019.03.011.
- Ananieva EA, Bostic JN, Torres AA, Glanz HR, McNitt SM, Brenner MK, Boyer MP, Addington AK, Hutson SM. (2018) Mice deficient in the mitochondrial branched-chain aminotransferase (BCATm) respond with delayed tumour growth to a challenge with EL-4 lymphoma. Br J Cancer. 2018 Oct;119(8):1009-1017. doi: 10.1038/s41416-018-0283-7.
Poster Presentations
- Adele K. Addington, Rebecca K. Mammel, Xiaoran Wei, Kalyn S. Specht, Jacob M. Bond, Sarah R. Donnelly, Michelle L. Olsen, Siobhan M. Craige (2023) Endothelial cells and myocytes from mouse gastrocnemius demonstrate differential gene expression in response to exercise. Vascular Biology Gordon Research Conference. Ventura, CA.